Effects of gingival fibroblasts transfected with human transform growth factor-beta1 gene on improving the periodontal tissue regeneration / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the effects of gingival fibroblasts (GF) transfected with hTGF-beta1 gene on improving the periodontal tissue regeneration for the repair of degree II artificial furcation defects.</p><p><b>METHODS</b>The gingival fibroblasts transfected with hTGF-beta1 gene was compounded to the cuttlebone-transformed nanometer hydroxyapatite (CBHA) material from the cuttlefish in vitro, the degree II furcation defects on the premolars of dogs were produced surgically, and the compound was to implanted into the defect (transfected group), and compared with the compound of periodontal ligament cells (PDLC) with nanometer HA material and the compound of untransfected GF with HA. The results were examined histologically 8 weeks after operation.</p><p><b>RESULTS</b>In the transfected group and the positive control group, more new attachment was found compared with the negative control (P < 0.01), and the NC, NB and NC of the transfected group and the positive control group were: (2.97 +/- 0.50), (4.29 +/- 0.26) and (4.73 +/- 0.06) mm; (3.09 +/- 0.26), (4.46 +/- 0.25) and (4.69 +/- 0.10) mm, respectively. There was no significant difference between the two groups (P > 0.05). Although the alveolar bone regeneration was found in the untransfected group [NB = (3.46 +/- 0.32) mm], the root resorption was observed. The tracing experiment showed that the transfected GF were found in the new alveolar bone and the periodontal membrane.</p><p><b>CONCLUSIONS</b>GF transfected with hTGF-beta1 gene can significantly improve the periodontal tissue regeneration in treatment of degree II furcation defects and is involved in the formation of the new alveolar bone and the new periodontal membrane.</p>

Preliminary report on measurement of human periodontal biologic width / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To observe and measure the biologic width in Chinese people.</p><p><b>METHODS</b>Jaws of three male Han Chinese cadavers were collected. All specimens were taken as autopsy as block sections. A total of 174 mesiodistal and buccolingual sections in 6 microm thickness were cut and stained with hematoxylin and eosin. The biologic width was measured under microscope with a micrometer at the middle of the mesial, distal, buccal, and lingual sides. Differences in biologic width were compared among 4 measurement sites, as well as among anterior tooth, premolar, and molar groups.</p><p><b>RESULTS</b>The mean of biologic width of measured teeth was 2.17 mm, with the average width of junctional epithelium being 1.07 mm and the average width of connective tissue being 1.10 mm. No statistic difference was found among 4 measured sites. The biologic width of premolars (2.23 mm) and molars (2.25 mm) was larger than that of anterior teeth (2.07 mm).</p><p><b>CONCLUSIONS</b>The average biologic width of three Chinese people is 2.17 mm. The biologic width of posterior teeth is larger than that of anterior teeth.</p>